there are four imperfect repeats v-v-e-k-k-n / e-e of which the core sequence is similar to map ib of mouse . rna blot and rt-pcr analysis showed that this gene is expressed specifically in the mature pollen and can be classified as a late gene in pollen development 計(jì)算機(jī)軟件分析st901基因核苷酸、氨基酸序列的相似性,結(jié)果表明,st901基因編碼區(qū)與探針sb401、與高賴氨酸基因sblr、與番茄tsb具有較高的相似性,它們可能來(lái)源于馬鈴薯的一個(gè)基因家族。
the two single-pass transmembrane proteins, delta and serrate, have been identified as notch ligands . the transcription factor suppressor of hairless [ su ( h ) ] is the major downstream effector of notch signaling pathway . rbp-j, the mammalian homolog of su ( h ), recognizes the core sequence ( c / tgtgggaa ) of dna rbp-j是果蠅促神經(jīng)發(fā)生基因su(h)(suppressorofhairless)在哺乳動(dòng)物的同源物,它通過(guò)其識(shí)別序列(ctgtgggaa)結(jié)合于受調(diào)控基因的啟動(dòng)子區(qū),在轉(zhuǎn)錄激活因子的驅(qū)動(dòng)下調(diào)節(jié)細(xì)胞分化和個(gè)體發(fā)育相關(guān)基因的表達(dá)。
through three rounds of screening, seventeen clones were selected and used in competitive test . the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera . based on the amin acid sequences deduced from the foreign sequence inserted in the phage, it was indicated that all clones shared the core sequence-p / ekphf, that was similar to aa50 ~ aa55 domain of n protein of prrsv 從第三輪親和篩選的噬菌體中隨機(jī)挑取17個(gè)克隆進(jìn)行功能鑒定,結(jié)果表明8個(gè)克隆與mabge3具有較強(qiáng)的特異性結(jié)合力并可以被prrsv陽(yáng)性血清阻斷,測(cè)序發(fā)現(xiàn)7個(gè)克隆具有核心序列:pekphf,該序列與prrsvn蛋白aa50aa55(pekphf)具有較高的同源性。